Warning: count(): Parameter must be an array or an object that implements Countable in /home/jobin/public_html/qa-theme/Donut-theme-m/qa-donut-layer.php on line 1250

Warning: count(): Parameter must be an array or an object that implements Countable in /home/jobin/public_html/qa-theme/Donut-theme-m/qa-donut-layer.php on line 1250

Warning: count(): Parameter must be an array or an object that implements Countable in /home/jobin/public_html/qa-theme/Donut-theme-m/qa-donut-layer.php on line 243

Warning: count(): Parameter must be an array or an object that implements Countable in /home/jobin/public_html/qa-theme/Donut-theme-m/qa-donut-layer.php on line 1250

 

Hello : ) This is Lakshmi from India. I have a doubt while doing isolation of DNA from a bacterial cell.We were given a protocol and it says that we have to resusupend bacterial pellet in SET buffer, then add SDS , then add NaCl, and then add NaI and 20% Isopropanol.Spin down the cells.To the supernatant add Silica and stay for a while and centrifuge it .Finally wash the pellet with 70% ethanol and resuspend the pellet in TE buffer.
 
I dont know how the RNA and Plasmid DNA is going away ?Can you please help me how to figure out this?
 
Thanks a lot :)
 
by

Your answer

Your name to display (optional):
Privacy: Your email address will only be used for sending these notifications.
Anti-spam verification:
To avoid this verification in future, please log in or register.

Warning: count(): Parameter must be an array or an object that implements Countable in /home/jobin/public_html/qa-theme/Donut-theme-m/qa-donut-layer.php on line 1250

Related questions

1 answer
0 answers
0 answers
asked Oct 3, 2013 by anonymous | 82 views
0 answers
asked Mar 8, 2012 by anonymous | 75 views

Warning: count(): Parameter must be an array or an object that implements Countable in /home/jobin/public_html/qa-theme/Donut-theme-m/qa-donut-layer.php on line 1250

Warning: count(): Parameter must be an array or an object that implements Countable in /home/jobin/public_html/qa-theme/Donut-theme-m/qa-donut-layer.php on line 1250
1,365 questions
1,128 answers
30 comments
171,983 users